In transgenic animal models. the conservation of DNA sequences between the transgene and the host wild-type gene can complicate the evaluation of the expression of each gene. The potential for gene silencing may complicate matters further. Here we report the use of RT-PCR heteroduplex analysis to differentiate the expression of a transgene and its homologous wild-type. https://countryscenesaddleryandpetsuppliers.shop/product-category/horse-wear-halters-bronc-halters/